Sequencing

Our technology platform offers comprehensive high-throughput, next-generation sequencing (NGS) for DNA and RNA. We focus our capabilities in the area of human cancers, with proven success in generating reliable results from low input and/or low quality biospecimens.

Our Sequencing Services & Resources

Our Sequencing Services

For studies needing the most comprehensive information about important samples, OICR can perform whole genome sequencing with as little as 50 nanograms of input DNA.

Standard coverage (30X)

Standard coverage whole genomes are adequate for most applications where variations are readily identifiable, such as germline SNVs or tumour cell lines with little heterogeneity. 

Applications: 

  • Germline mutations
  • Structural variants

Minimum: 50ng DNA

Deep coverage (>50X)

Deep coverage genomes provide a more detailed view of DNA when cellular heterogenity is high, or where complicated structural changes such as chromothripsis are expected.

Applications: 

  • Rare somatic mutations
  • Complex structural variants

Minimum: 100ng DNA

Transcriptomes

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For insights into cellular activities, transcriptomes offer a dynamic view. Between whole transcriptomes, RNA exomes/transcriptome capture and targeted transcriptomes, we can match the right kit to your application. We have extensive experience creating transcriptomes from both intact and highly degraded FFPE materials.

Exomes and other targeted panels

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Our group has delivered on demanding targeted projects from tens to hundreds of samples, including both off-the-shelf and custom panels for genomes and cell-free DNA

Long Read

As opposed to Illumina sequencing, which generates ~150bp reads, Long Read Sequencing with Oxford Nanopore produces reads from 1,000 to 1,000,000+ bp. Long Read technology is ideally suited for whole genome mutational, structural, and epigenetic variation, as well as to support comprehensive transcriptome analyses

cfMeDIP

Cell Free DNA methyl-Seq (cfMeDIP) is an emerging methylome sequencing technology to support epigenetic projects in the cell free space.

SARS-CoV-2 Sequencing

Over 3,500 viral genomes sequenced

Tissue Extraction

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We perform nucleic acid extraction from buffy coat, FFPE and fresh frozen tissues.

Sequencing-only services

If you have already prepared libraries, we can sequence them for you.

Customized Sequencing

If off-the-shelf solutions won’t fulfill your project needs, our team can create something new for you. We have helped dozens of researchers develop complex, customized genomics and analysis plans for medium and large-scale projects.

Our Technology Platforms

Our sequencing suite, located in downtown Toronto, Ontario, includes the following platforms:

  • Illumina NovaSeq 6000
  • Illumina HiSeq 2500
  • Illumina MiSeq
  • Illumina NextSeq 550
  • Oxford Nanopore MinION

How to work with us

Our manuals can help you with important information on our workflows and policies:

  • Sample Requirements and Submission
  • Library Preparation and Sequencing Protocols
  • Our Terms & Conditions and Acknowledgement Policy