Sequencing

Our technology platform offers comprehensive high-throughput, next-generation sequencing (NGS) for DNA and RNA. We focus our capabilities in the area of human cancers, with proven success in generating reliable results from low input and/or low quality biospecimens.

Our Sequencing Services & Resources

Our Sequencing Services

Tissue Extraction

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We can help perform nucleic acid extraction from buffy coat, FFPE and fresh frozen tissues.

For studies needing the most comprehensive information about important samples, OICR can perform whole genome sequencing with as little as 50 nanograms of input DNA.

Standard coverage (30X)

Standard coverage whole genomes are adequate for most applications where variations are readily identifiable, such as germline SNVs or tumour cell lines with little heterogeneity. 

Applications: 

  • Germline mutations
  • Structural variants

Minimum: 50ng DNA

Deep coverage (>50X)

Deep coverage genomes provide a more detailed view of DNA when cellular heterogenity is high, or where complicated structural changes such as chromothripsis are expected.

Applications: 

  • Rare somatic mutations
  • Complex structural variants

Minimum: 100ng DNA

Transcriptomes

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For insights into cellular activities, transcriptomes offer a dynamic view. Between whole transcriptomes, RNA exomes/transcriptome capture and targeted transcriptomes, we can match the right kit to your application. We have extensive experience creating transcriptomes from both intact and highly degraded FFPE materials.

Exomes

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Our group has delivered on demanding exome projects from those involving hundreds of samples down to unique cases requiring exomes from only 100ng of DNA. Our in-house expertise can construct exomes from many types of samples, from abundant cell lines all the way to the most degraded archival specimens you can find.

Targeted Panels

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Targeted sequencing is a more economic alternative that we offer for deeper sequencing of clinically-relevant genes.

Long Read

As opposed to Illumina sequencing, which generates ~150bp reads, Long Read Sequencing produces reads from 1,000 to 1,000,000+ bp. Long Read technology is ideally suited for identifying chromosomal rearrangements, gene fusions, splicing isoforms, and post-transcriptional modifications.

Single-Cell

10X Genomics high-throughput scRNA-seq technology provides single cell expression measurements that enable discovery of gene expression dynamics and molecular profiling of individual cell types.

Customized Sequencing

When off-the-shelf solutions won’t fulfill your project needs, a dedicated genomics team can create something new for you. We have helped dozens of researchers develop complex, customized genomics and analysis plans that squeeze more out of any size of project.

Our Technology Platforms

Our sequencing suite, located in downtown Toronto, Ontario, includes the following platforms:

  • Illumina NovaSeq 6000
  • Illumina HiSeq 2500
  • Illumina MiSeq
  • Illumina NextSeq 550
  • Oxford Nanopore MinION

How to work with us

Our manuals can help you with important information on our workflows and policies:

  • Sample Requirements and Submission
  • Library Preparation and Sequencing Protocols
  • Our Terms & Conditions and Acknowledgement Policy